data acquisition card daq model 626 Search Results


96
Carl Zeiss axiovert 200m
Phagocytotic activity and upregulation of macrophage markers in VSMCs induced by MT-III. VSMCs (2.5 × 10 3 cells/well) were treated with MT-III (0.4 and 0.8 μM) or DMEM (Control) for 12, 24, and 48 h, followed by incubation with 0.5 μm fluorescent beads (red) for 6 h. Cells were then washed extensively, fixed with paraformaldehyde (4%), counterstained with Hoechst 33342 (blue-nuclei), and analyzed with an automated <t>Axiovert</t> 200 m followed by a computerized automatic quantification in the CellProfiler analysis software. ( A ) The number of latex beads per cell was counted to obtain numerical data for the total phagocytotic activity. ( B ) A representative field of view. ( C ) The effect of MT-III on VSMC and macrophage-related gene expression in rat VSMCs. Subconfluent rat VSMCs (8 × 10 5 cells/well) were treated with MT-III (0.4 and 0.8 μM) or with DMEM medium (control) for 48 h. Total RNA was extracted and subjected to RT-PCR analysis. Values represent the mean ± SEM from six animals ( n = 6) (ANOVA). Note: * p < 0.05 compared with control cells.
Axiovert 200m, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss axioscan.z1 fluorescence slide scanner
Phagocytotic activity and upregulation of macrophage markers in VSMCs induced by MT-III. VSMCs (2.5 × 10 3 cells/well) were treated with MT-III (0.4 and 0.8 μM) or DMEM (Control) for 12, 24, and 48 h, followed by incubation with 0.5 μm fluorescent beads (red) for 6 h. Cells were then washed extensively, fixed with paraformaldehyde (4%), counterstained with Hoechst 33342 (blue-nuclei), and analyzed with an automated <t>Axiovert</t> 200 m followed by a computerized automatic quantification in the CellProfiler analysis software. ( A ) The number of latex beads per cell was counted to obtain numerical data for the total phagocytotic activity. ( B ) A representative field of view. ( C ) The effect of MT-III on VSMC and macrophage-related gene expression in rat VSMCs. Subconfluent rat VSMCs (8 × 10 5 cells/well) were treated with MT-III (0.4 and 0.8 μM) or with DMEM medium (control) for 48 h. Total RNA was extracted and subjected to RT-PCR analysis. Values represent the mean ± SEM from six animals ( n = 6) (ANOVA). Note: * p < 0.05 compared with control cells.
Axioscan.Z1 Fluorescence Slide Scanner, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss zen blue 2012 software
Phagocytotic activity and upregulation of macrophage markers in VSMCs induced by MT-III. VSMCs (2.5 × 10 3 cells/well) were treated with MT-III (0.4 and 0.8 μM) or DMEM (Control) for 12, 24, and 48 h, followed by incubation with 0.5 μm fluorescent beads (red) for 6 h. Cells were then washed extensively, fixed with paraformaldehyde (4%), counterstained with Hoechst 33342 (blue-nuclei), and analyzed with an automated <t>Axiovert</t> 200 m followed by a computerized automatic quantification in the CellProfiler analysis software. ( A ) The number of latex beads per cell was counted to obtain numerical data for the total phagocytotic activity. ( B ) A representative field of view. ( C ) The effect of MT-III on VSMC and macrophage-related gene expression in rat VSMCs. Subconfluent rat VSMCs (8 × 10 5 cells/well) were treated with MT-III (0.4 and 0.8 μM) or with DMEM medium (control) for 48 h. Total RNA was extracted and subjected to RT-PCR analysis. Values represent the mean ± SEM from six animals ( n = 6) (ANOVA). Note: * p < 0.05 compared with control cells.
Zen Blue 2012 Software, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss libra 120 plus tem
Phagocytotic activity and upregulation of macrophage markers in VSMCs induced by MT-III. VSMCs (2.5 × 10 3 cells/well) were treated with MT-III (0.4 and 0.8 μM) or DMEM (Control) for 12, 24, and 48 h, followed by incubation with 0.5 μm fluorescent beads (red) for 6 h. Cells were then washed extensively, fixed with paraformaldehyde (4%), counterstained with Hoechst 33342 (blue-nuclei), and analyzed with an automated <t>Axiovert</t> 200 m followed by a computerized automatic quantification in the CellProfiler analysis software. ( A ) The number of latex beads per cell was counted to obtain numerical data for the total phagocytotic activity. ( B ) A representative field of view. ( C ) The effect of MT-III on VSMC and macrophage-related gene expression in rat VSMCs. Subconfluent rat VSMCs (8 × 10 5 cells/well) were treated with MT-III (0.4 and 0.8 μM) or with DMEM medium (control) for 48 h. Total RNA was extracted and subjected to RT-PCR analysis. Values represent the mean ± SEM from six animals ( n = 6) (ANOVA). Note: * p < 0.05 compared with control cells.
Libra 120 Plus Tem, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss lsm 510 software
Phagocytotic activity and upregulation of macrophage markers in VSMCs induced by MT-III. VSMCs (2.5 × 10 3 cells/well) were treated with MT-III (0.4 and 0.8 μM) or DMEM (Control) for 12, 24, and 48 h, followed by incubation with 0.5 μm fluorescent beads (red) for 6 h. Cells were then washed extensively, fixed with paraformaldehyde (4%), counterstained with Hoechst 33342 (blue-nuclei), and analyzed with an automated <t>Axiovert</t> 200 m followed by a computerized automatic quantification in the CellProfiler analysis software. ( A ) The number of latex beads per cell was counted to obtain numerical data for the total phagocytotic activity. ( B ) A representative field of view. ( C ) The effect of MT-III on VSMC and macrophage-related gene expression in rat VSMCs. Subconfluent rat VSMCs (8 × 10 5 cells/well) were treated with MT-III (0.4 and 0.8 μM) or with DMEM medium (control) for 48 h. Total RNA was extracted and subjected to RT-PCR analysis. Values represent the mean ± SEM from six animals ( n = 6) (ANOVA). Note: * p < 0.05 compared with control cells.
Lsm 510 Software, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss zen blue (zeiss lsm 800 confocal microscope image acquisition)
Phagocytotic activity and upregulation of macrophage markers in VSMCs induced by MT-III. VSMCs (2.5 × 10 3 cells/well) were treated with MT-III (0.4 and 0.8 μM) or DMEM (Control) for 12, 24, and 48 h, followed by incubation with 0.5 μm fluorescent beads (red) for 6 h. Cells were then washed extensively, fixed with paraformaldehyde (4%), counterstained with Hoechst 33342 (blue-nuclei), and analyzed with an automated <t>Axiovert</t> 200 m followed by a computerized automatic quantification in the CellProfiler analysis software. ( A ) The number of latex beads per cell was counted to obtain numerical data for the total phagocytotic activity. ( B ) A representative field of view. ( C ) The effect of MT-III on VSMC and macrophage-related gene expression in rat VSMCs. Subconfluent rat VSMCs (8 × 10 5 cells/well) were treated with MT-III (0.4 and 0.8 μM) or with DMEM medium (control) for 48 h. Total RNA was extracted and subjected to RT-PCR analysis. Values represent the mean ± SEM from six animals ( n = 6) (ANOVA). Note: * p < 0.05 compared with control cells.
Zen Blue (Zeiss Lsm 800 Confocal Microscope Image Acquisition), supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Carl Zeiss zeiss imager.a2 microscope
Phagocytotic activity and upregulation of macrophage markers in VSMCs induced by MT-III. VSMCs (2.5 × 10 3 cells/well) were treated with MT-III (0.4 and 0.8 μM) or DMEM (Control) for 12, 24, and 48 h, followed by incubation with 0.5 μm fluorescent beads (red) for 6 h. Cells were then washed extensively, fixed with paraformaldehyde (4%), counterstained with Hoechst 33342 (blue-nuclei), and analyzed with an automated <t>Axiovert</t> 200 m followed by a computerized automatic quantification in the CellProfiler analysis software. ( A ) The number of latex beads per cell was counted to obtain numerical data for the total phagocytotic activity. ( B ) A representative field of view. ( C ) The effect of MT-III on VSMC and macrophage-related gene expression in rat VSMCs. Subconfluent rat VSMCs (8 × 10 5 cells/well) were treated with MT-III (0.4 and 0.8 μM) or with DMEM medium (control) for 48 h. Total RNA was extracted and subjected to RT-PCR analysis. Values represent the mean ± SEM from six animals ( n = 6) (ANOVA). Note: * p < 0.05 compared with control cells.
Zeiss Imager.A2 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Advantech multifunction data acquisition card
Phagocytotic activity and upregulation of macrophage markers in VSMCs induced by MT-III. VSMCs (2.5 × 10 3 cells/well) were treated with MT-III (0.4 and 0.8 μM) or DMEM (Control) for 12, 24, and 48 h, followed by incubation with 0.5 μm fluorescent beads (red) for 6 h. Cells were then washed extensively, fixed with paraformaldehyde (4%), counterstained with Hoechst 33342 (blue-nuclei), and analyzed with an automated <t>Axiovert</t> 200 m followed by a computerized automatic quantification in the CellProfiler analysis software. ( A ) The number of latex beads per cell was counted to obtain numerical data for the total phagocytotic activity. ( B ) A representative field of view. ( C ) The effect of MT-III on VSMC and macrophage-related gene expression in rat VSMCs. Subconfluent rat VSMCs (8 × 10 5 cells/well) were treated with MT-III (0.4 and 0.8 μM) or with DMEM medium (control) for 48 h. Total RNA was extracted and subjected to RT-PCR analysis. Values represent the mean ± SEM from six animals ( n = 6) (ANOVA). Note: * p < 0.05 compared with control cells.
Multifunction Data Acquisition Card, supplied by Advantech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Chrom Tech chrom card trace focus gc software
Phagocytotic activity and upregulation of macrophage markers in VSMCs induced by MT-III. VSMCs (2.5 × 10 3 cells/well) were treated with MT-III (0.4 and 0.8 μM) or DMEM (Control) for 12, 24, and 48 h, followed by incubation with 0.5 μm fluorescent beads (red) for 6 h. Cells were then washed extensively, fixed with paraformaldehyde (4%), counterstained with Hoechst 33342 (blue-nuclei), and analyzed with an automated <t>Axiovert</t> 200 m followed by a computerized automatic quantification in the CellProfiler analysis software. ( A ) The number of latex beads per cell was counted to obtain numerical data for the total phagocytotic activity. ( B ) A representative field of view. ( C ) The effect of MT-III on VSMC and macrophage-related gene expression in rat VSMCs. Subconfluent rat VSMCs (8 × 10 5 cells/well) were treated with MT-III (0.4 and 0.8 μM) or with DMEM medium (control) for 48 h. Total RNA was extracted and subjected to RT-PCR analysis. Values represent the mean ± SEM from six animals ( n = 6) (ANOVA). Note: * p < 0.05 compared with control cells.
Chrom Card Trace Focus Gc Software, supplied by Chrom Tech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
POWERLAB INC 4/20 data recording system
Phagocytotic activity and upregulation of macrophage markers in VSMCs induced by MT-III. VSMCs (2.5 × 10 3 cells/well) were treated with MT-III (0.4 and 0.8 μM) or DMEM (Control) for 12, 24, and 48 h, followed by incubation with 0.5 μm fluorescent beads (red) for 6 h. Cells were then washed extensively, fixed with paraformaldehyde (4%), counterstained with Hoechst 33342 (blue-nuclei), and analyzed with an automated <t>Axiovert</t> 200 m followed by a computerized automatic quantification in the CellProfiler analysis software. ( A ) The number of latex beads per cell was counted to obtain numerical data for the total phagocytotic activity. ( B ) A representative field of view. ( C ) The effect of MT-III on VSMC and macrophage-related gene expression in rat VSMCs. Subconfluent rat VSMCs (8 × 10 5 cells/well) were treated with MT-III (0.4 and 0.8 μM) or with DMEM medium (control) for 48 h. Total RNA was extracted and subjected to RT-PCR analysis. Values represent the mean ± SEM from six animals ( n = 6) (ANOVA). Note: * p < 0.05 compared with control cells.
4/20 Data Recording System, supplied by POWERLAB INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Becker & Hickl spc 850 data acquisition card
Phagocytotic activity and upregulation of macrophage markers in VSMCs induced by MT-III. VSMCs (2.5 × 10 3 cells/well) were treated with MT-III (0.4 and 0.8 μM) or DMEM (Control) for 12, 24, and 48 h, followed by incubation with 0.5 μm fluorescent beads (red) for 6 h. Cells were then washed extensively, fixed with paraformaldehyde (4%), counterstained with Hoechst 33342 (blue-nuclei), and analyzed with an automated <t>Axiovert</t> 200 m followed by a computerized automatic quantification in the CellProfiler analysis software. ( A ) The number of latex beads per cell was counted to obtain numerical data for the total phagocytotic activity. ( B ) A representative field of view. ( C ) The effect of MT-III on VSMC and macrophage-related gene expression in rat VSMCs. Subconfluent rat VSMCs (8 × 10 5 cells/well) were treated with MT-III (0.4 and 0.8 μM) or with DMEM medium (control) for 48 h. Total RNA was extracted and subjected to RT-PCR analysis. Values represent the mean ± SEM from six animals ( n = 6) (ANOVA). Note: * p < 0.05 compared with control cells.
Spc 850 Data Acquisition Card, supplied by Becker & Hickl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sensoray Co Inc data card sensoray model 626
Phagocytotic activity and upregulation of macrophage markers in VSMCs induced by MT-III. VSMCs (2.5 × 10 3 cells/well) were treated with MT-III (0.4 and 0.8 μM) or DMEM (Control) for 12, 24, and 48 h, followed by incubation with 0.5 μm fluorescent beads (red) for 6 h. Cells were then washed extensively, fixed with paraformaldehyde (4%), counterstained with Hoechst 33342 (blue-nuclei), and analyzed with an automated <t>Axiovert</t> 200 m followed by a computerized automatic quantification in the CellProfiler analysis software. ( A ) The number of latex beads per cell was counted to obtain numerical data for the total phagocytotic activity. ( B ) A representative field of view. ( C ) The effect of MT-III on VSMC and macrophage-related gene expression in rat VSMCs. Subconfluent rat VSMCs (8 × 10 5 cells/well) were treated with MT-III (0.4 and 0.8 μM) or with DMEM medium (control) for 48 h. Total RNA was extracted and subjected to RT-PCR analysis. Values represent the mean ± SEM from six animals ( n = 6) (ANOVA). Note: * p < 0.05 compared with control cells.
Data Card Sensoray Model 626, supplied by Sensoray Co Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Phagocytotic activity and upregulation of macrophage markers in VSMCs induced by MT-III. VSMCs (2.5 × 10 3 cells/well) were treated with MT-III (0.4 and 0.8 μM) or DMEM (Control) for 12, 24, and 48 h, followed by incubation with 0.5 μm fluorescent beads (red) for 6 h. Cells were then washed extensively, fixed with paraformaldehyde (4%), counterstained with Hoechst 33342 (blue-nuclei), and analyzed with an automated Axiovert 200 m followed by a computerized automatic quantification in the CellProfiler analysis software. ( A ) The number of latex beads per cell was counted to obtain numerical data for the total phagocytotic activity. ( B ) A representative field of view. ( C ) The effect of MT-III on VSMC and macrophage-related gene expression in rat VSMCs. Subconfluent rat VSMCs (8 × 10 5 cells/well) were treated with MT-III (0.4 and 0.8 μM) or with DMEM medium (control) for 48 h. Total RNA was extracted and subjected to RT-PCR analysis. Values represent the mean ± SEM from six animals ( n = 6) (ANOVA). Note: * p < 0.05 compared with control cells.

Journal: Molecules

Article Title: A Secreted Phospholipase A 2 Induces Formation of Smooth Muscle Foam Cells Which Transdifferentiate to Macrophage-Like State

doi: 10.3390/molecules24183244

Figure Lengend Snippet: Phagocytotic activity and upregulation of macrophage markers in VSMCs induced by MT-III. VSMCs (2.5 × 10 3 cells/well) were treated with MT-III (0.4 and 0.8 μM) or DMEM (Control) for 12, 24, and 48 h, followed by incubation with 0.5 μm fluorescent beads (red) for 6 h. Cells were then washed extensively, fixed with paraformaldehyde (4%), counterstained with Hoechst 33342 (blue-nuclei), and analyzed with an automated Axiovert 200 m followed by a computerized automatic quantification in the CellProfiler analysis software. ( A ) The number of latex beads per cell was counted to obtain numerical data for the total phagocytotic activity. ( B ) A representative field of view. ( C ) The effect of MT-III on VSMC and macrophage-related gene expression in rat VSMCs. Subconfluent rat VSMCs (8 × 10 5 cells/well) were treated with MT-III (0.4 and 0.8 μM) or with DMEM medium (control) for 48 h. Total RNA was extracted and subjected to RT-PCR analysis. Values represent the mean ± SEM from six animals ( n = 6) (ANOVA). Note: * p < 0.05 compared with control cells.

Article Snippet: Data acquisition was accomplished with automated Axiovert 200m (Carl Zeiss MicroImaging GmbH, Jena, Germany) followed by a computerized automatic quantification in the CellProfiler analysis software.

Techniques: Activity Assay, Incubation, Software, Expressing, Reverse Transcription Polymerase Chain Reaction