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Image Search Results
Journal: Molecules
Article Title: A Secreted Phospholipase A 2 Induces Formation of Smooth Muscle Foam Cells Which Transdifferentiate to Macrophage-Like State
doi: 10.3390/molecules24183244
Figure Lengend Snippet: Phagocytotic activity and upregulation of macrophage markers in VSMCs induced by MT-III. VSMCs (2.5 × 10 3 cells/well) were treated with MT-III (0.4 and 0.8 μM) or DMEM (Control) for 12, 24, and 48 h, followed by incubation with 0.5 μm fluorescent beads (red) for 6 h. Cells were then washed extensively, fixed with paraformaldehyde (4%), counterstained with Hoechst 33342 (blue-nuclei), and analyzed with an automated Axiovert 200 m followed by a computerized automatic quantification in the CellProfiler analysis software. ( A ) The number of latex beads per cell was counted to obtain numerical data for the total phagocytotic activity. ( B ) A representative field of view. ( C ) The effect of MT-III on VSMC and macrophage-related gene expression in rat VSMCs. Subconfluent rat VSMCs (8 × 10 5 cells/well) were treated with MT-III (0.4 and 0.8 μM) or with DMEM medium (control) for 48 h. Total RNA was extracted and subjected to RT-PCR analysis. Values represent the mean ± SEM from six animals ( n = 6) (ANOVA). Note: * p < 0.05 compared with control cells.
Article Snippet: Data acquisition was accomplished with automated
Techniques: Activity Assay, Incubation, Software, Expressing, Reverse Transcription Polymerase Chain Reaction